1QU2
INSIGHTS INTO EDITING FROM AN ILE-TRNA SYNTHETASE STRUCTURE WITH TRNA(ILE) AND MUPIROCIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | PRINCETON 2K |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.000, 100.000, 186.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.28100 |
RMSD bond length | 0.008 |
RMSD bond angle | 21.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 500.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.079 * |
Number of reflections | 67758 |
Completeness [%] | 89.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 20 * | EVAPORATION |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | IleRS | 0.050 (mM) | |
2 | 1 | drop | tRNA | 0.050 (mM) | |
3 | 1 | drop | Mupirocin | 1 (mM) | |
4 | 1 | reservoir | PEG6000 | 12 (%) | |
5 | 1 | reservoir | 0.3 (M) | ||
6 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
7 | 1 | reservoir | 100 (mM) | ||
8 | 1 | reservoir | 2 (mM) | ||
9 | 1 | reservoir | beta-octyl glucopyranoside | 0.1 (%) |