1QU1

CRYSTAL STRUCTURE OF EHA2 (23-185)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	1998-01-12
Detector	FUJI
Spacegroup name	C 1 2 1
Unit cell lengths	113.241, 48.877, 221.303
Unit cell angles	90.00, 103.26, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.229
Rwork	0.229
R-free	0.25000
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.090	0.277
Number of reflections	87961	7801 *
<I/σ(I)>	7.7
Completeness [%]	94.2	83.9
Redundancy	2.6	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		2% PEG 4000, 30-150 MM NACL, pH 4.40

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	reservoir	PEG4000	2 (%)
4	1	reservoir		30-150 (mM)