1QTZ

D20C MUTANT OF T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Detector technology	AREA DETECTOR
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 32 2 1
Unit cell lengths	61.050, 61.050, 97.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.165
RMSD bond length	0.019
RMSD bond angle	2.900
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.050
High resolution limit [Å]	1.900	1.900
Rmerge	0.044 *	0.185
Total number of observations	28398 *
Number of reflections	15116
<I/σ(I)>	8
Completeness [%]	87.0	80
Redundancy	1.9	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *			Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	phosphate	2.0 (M)
3	1	reservoir		0.25 (M)
4	1	reservoir	beta-mercaptoethanol