1QTY
VASCULAR ENDOTHELIAL GROWTH FACTOR IN COMPLEX WITH DOMAIN 2 OF THE FLT-1 RECEPTOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1996-12-24 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.310, 67.010, 120.840 |
Unit cell angles | 90.00, 118.15, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.2327 |
Rwork | 0.233 |
R-free | 0.28440 |
Structure solution method | MOLECULAR REPLACEMENT IN COMBINATION WITH MULTI-CRYSTAL AVERAGING |
Starting model (for MR) | 1vpf |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.760 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 24098 | |
<I/σ(I)> | 13.3 | |
Completeness [%] | 99.5 | 98 |
Redundancy | 3.7 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | Wiesmann, C., (1997) Cell, 91, 695. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | reservoir | PEG4000 | 30 (%) | |
4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
5 | 1 | reservoir | 0.02 (%) | ||
6 | 1 | reservoir | Tris-HCl | 0.15 (M) |