1QTS
CRYSTAL STRUCTURE OF THE AP-2 CLATHRIN ADAPTOR ALPHA-APPENDAGE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1998-12-05 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.807, 72.749, 41.861 |
| Unit cell angles | 90.00, 99.69, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| Rwork | 0.178 |
| R-free | 0.20900 |
| RMSD bond length | 0.001 * |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.100 | 0.672 * |
| Total number of observations | 495848 * | |
| Number of reflections | 45711 | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 96.1 | 82.1 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 20 * | 1.3 M AMMONIUM SULFATE, 80 MM HEPES, 8% DIOXANE, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.3 (M) | |
| 3 | 1 | reservoir | HEPES | 80 (mM) | |
| 4 | 1 | reservoir | dioxane | 8 (%) |
