1QTS

CRYSTAL STRUCTURE OF THE AP-2 CLATHRIN ADAPTOR ALPHA-APPENDAGE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	110
Detector technology	CCD
Collection date	1998-12-05
Detector	CUSTOM-MADE
Spacegroup name	P 1 21 1
Unit cell lengths	40.807, 72.749, 41.861
Unit cell angles	90.00, 99.69, 90.00

Refinement procedure

Resolution	20.000 - 1.400
Rwork	0.178
R-free	0.20900
RMSD bond length	0.001 *
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.100	0.672 *
Total number of observations	495848 *
Number of reflections	45711
<I/σ(I)>	23
Completeness [%]	96.1	82.1
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	20 *	1.3 M AMMONIUM SULFATE, 80 MM HEPES, 8% DIOXANE, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	ammonium sulfate	1.3 (M)
3	1	reservoir	HEPES	80 (mM)
4	1	reservoir	dioxane	8 (%)