1QTK

CRYSTAL STRUCTURE OF HEW LYSOZYME UNDER PRESSURE OF KRYPTON (55 BAR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-02-10
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	78.530, 78.530, 37.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.900 - 2.030
R-factor	0.1698
R-free	0.21600
RMSD bond length	0.006
RMSD bond angle	0.023
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.900	2.150
High resolution limit [Å]	2.030	2.030
Rmerge	0.057	0.141
Number of reflections	7734
<I/σ(I)>	9.3
Completeness [%]	80.0	63
Redundancy	4.8	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5 *	20 *	Sodium chloride , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 290.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	elastase	2 (%)
2	1	1	sodium acetate	0.01 (M)
3	1	1	sodium sulfate	0.02 (M)