1QTD

THE INTRODUCTION OF STRAIN AND ITS EFFECTS ON THE STRUCTURE AND STABILITY OF T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1997-03-01
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 32 2 1
Unit cell lengths	60.968, 60.968, 97.002
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.500
Rwork	0.168
RMSD bond length	0.016
RMSD bond angle	18.859 *
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.300	2.655
High resolution limit [Å]	2.500	2.500
Rmerge	0.057	0.212
Number of reflections	8267
<I/σ(I)>	8.066
Completeness [%]	93.0 *	65.3 *
Redundancy	3.16	1.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	277	seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	phosphate	2.0 (M)
3	1	reservoir		0.25 (M)
4	1	reservoir	oxidized beta-mercaptoethanol