1QT6

E11H Mutant of T4 Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Detector technology	IMAGE PLATE
Spacegroup name	P 32 2 1
Unit cell lengths	61.080, 61.080, 96.760
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.165
RMSD bond length	0.013
RMSD bond angle	2.600
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.000	2.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.063
Total number of observations	40327 *
Number of reflections	17643
Completeness [%]	87.0	72
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7 *		Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	phosphate	2.0 (M)
3	1	reservoir		0.25 (M)
4	1	reservoir	beta-mercaptoethanol