1QT1
CRYSTAL STRUCTURE OF XYLOSE ISOMERASE FROM STREPTOMYCES DIASTATICUS NO.7 M1033 AT 1.85 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.976, 98.836, 93.927 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.850 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1clk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.567 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 5.000 | 1.930 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.085 | |
| Number of reflections | 55255 | |
| <I/σ(I)> | 10.95 | 3.45 |
| Completeness [%] | 82.8 | 72.8 * |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.02 (M) | |
| 3 | 1 | drop | ammonium sulfate | 1.0 (M) | |
| 4 | 1 | drop | 0.01 (M) | ||
| 5 | 1 | drop | 0.001 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 0.02 (M) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.0 (M) |
