1QSU
CRYSTAL STRUCTURE OF THE TRIPLE-HELICAL COLLAGEN-LIKE PEPTIDE, (PRO-HYP-GLY)4-GLU-LYS-GLY(PRO-HYP-GLY)5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1993-10-15 |
| Detector | ENRAF-NONIUS CAD4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.326, 26.573, 45.885 |
| Unit cell angles | 90.00, 96.04, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.18 * |
| Rwork | 0.180 |
| R-free | 0.22700 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.240 * |
| Data reduction software | CAD4 |
| Data scaling software | MOLEN |
| Phasing software | MERLOT |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.810 | |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 5745 * | |
| Completeness [%] | 100.0 | 99.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | PEG 4K, tris HCl, Li2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 10 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 15 (%) | |
| 3 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 4 | 1 | drop | 0.3 (M) | ||
| 5 | 1 | reservoir | Tris | 0.1 (M) | |
| 6 | 1 | reservoir | 0.6 (M) | ||
| 7 | 1 | reservoir | PEG4000 | 30 (%) |
