1QST
CRYSTAL STRUCTURE OF TETRAHYMENA GCN5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-04-29 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.100, 64.100, 97.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 1.700 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.27800 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.292 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASES |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.780 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.045 | 0.149 |
Total number of observations | 497452 * | |
Number of reflections | 25427 * | |
<I/σ(I)> | 9.4 | |
Completeness [%] | 97.3 | 86.3 |
Redundancy | 19.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | LITHIUM SULFATE, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | LITHIUM SULFATE, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.5 (mg/ml) | |
2 | 1 | drop | 0.5 (M) | ||
3 | 1 | drop | HEPES | 40 (mM) | |
4 | 1 | reservoir | 1.25 (M) | ||
5 | 1 | reservoir | HEPES | 100 (mM) |