1QSR
CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND ACETYL-COENZYME A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-07 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.380, 64.380, 97.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.26100 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.335 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.054 | 0.182 |
Total number of observations | 158305 * | |
Number of reflections | 16255 | |
<I/σ(I)> | 21.1 | |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 9.7 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 25 * | HEPES, AMMONIUM SULFATE, MAGNESIUM SULFATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
2 | 1 | drop | acetyl-CoA | 1.5 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
4 | 1 | reservoir | HEPES | 50 (mM) | |
5 | 1 | reservoir | 50 (mM) |