1QSP
CRYSTAL STRUCTURE OF THE YEAST PHOSPHORELAY PROTEIN YPD1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11-20 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 52.710, 52.710, 244.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.206 |
Rwork | 0.201 |
R-free | 0.25400 |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.664 |
Data reduction software | XFIT |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.066 | 0.078 |
Number of reflections | 9674 * | |
<I/σ(I)> | 9.7 | |
Completeness [%] | 91.6 | 82 |
Redundancy | 5.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | Xu, Q., (1999) Acta Crystallogr., Sect.D, 55, 291. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 30-33 (%(w/v)) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
3 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
4 | 1 | drop | protein | 10-12 (mg/ml) |