1QSJ
N-TERMINALLY TRUNCATED C3DG FRAGMENT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.620, 99.930, 114.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.000 - 1.900 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.25300 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.940 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.170 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.045 | 0.083 |
| Number of reflections | 64362 | 18274 * |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 76.0 | 77.1 |
| Redundancy | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 15 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.01 (M) | |
| 3 | 1 | reservoir | PEG6000 | 25-30 (%(w/v)) | |
| 4 | 1 | reservoir | potassium phosphate | 0.05 (M) |
