1QSE
STRUCTURE OF HUMAN A6-TCR BOUND TO HLA-A2 COMPLEXED WITH ALTERED HTLV-1 TAX PEPTIDE V7R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-13 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11-25 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 224.862, 48.891, 94.456 |
Unit cell angles | 90.00, 90.49, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.800 |
Rwork | 0.243 |
R-free | 0.29000 |
RMSD bond length | 0.080 * |
RMSD bond angle | 1.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.071 | 0.530 |
Number of reflections | 23577 | |
<I/σ(I)> | 12.2 | |
Completeness [%] | 91.6 * | 60.8 * |
Redundancy | 2.1 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 * | 290 | seeding with hanging drop vapor diffusion; 50 mM MOPS, pH 7.0, 75 mM MgSO4 and 13% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 12 (mg/ml) | |
2 | 1 | 1 | HLA-A2/peptide complexes | ||
3 | 1 | 1 | MES | 10 (mM) | |
4 | 1 | 1 | 50 (mM) | ||
5 | 1 | 1 | EDTA | 0.5 (mM) |