1QSD

RBL2P, BETA-TUBULIN BINDING POST-CHAPERONIN COFACTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	289
Detector technology	AREA DETECTOR
Collection date	1998-01-01
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	45.400, 110.100, 49.400
Unit cell angles	90.00, 106.20, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.211
Rwork	0.208
R-free	0.29700
RMSD bond length	0.010
RMSD bond angle	1.668
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	SHELXS
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.061	0.152
Number of reflections	11128
<I/σ(I)>	12.8
Completeness [%]	96.2	96.1
Redundancy	2.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	293	1.6 M NA-CITRATE/CITRIC ACID, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	trisodium citrate/citric acid	1.6 (M)