1QS7
The 1.8 angstrom structure of calmodulin rs20 peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 107 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-07 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.016, 54.481, 66.304 |
| Unit cell angles | 90.00, 92.76, 90.00 |
Refinement procedure
| Resolution | 28.870 - 1.800 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vrk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.870 | 1.840 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.042 | 0.160 |
| Number of reflections | 32557 | |
| <I/σ(I)> | 24 | 8.6 |
| Completeness [%] | 82.8 | 91.8 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 20% (W/V) PEG 8000, 50 MM SODIUM ACETATE 5 MM CALCIUM CHLORIDE 0.01% (W/V) SODIUM AZIDE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
