1QS5
THE INTRODUCTION OF STRAIN AND ITS EFFECTS ON THE STRUCTURE AND STABILITY OF T4 LYSOZYME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.949, 60.949, 96.999 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.500 |
Rwork | 0.129 |
RMSD bond length | 0.015 |
RMSD bond angle | 18.494 * |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.500 | 2.760 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.075 | 0.185 |
Number of reflections | 7507 | |
<I/σ(I)> | 6.039 | |
Completeness [%] | 78.0 * | 70 * |
Redundancy | 4.86 | 2.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 277 | seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | phosphate | 2.0 (M) | |
3 | 1 | reservoir | 0.25 (M) | ||
4 | 1 | reservoir | oxidized beta-mercaptoethanol |