1QS1
CRYSTAL STRUCTURE OF VEGETATIVE INSECTICIDAL PROTEIN2 (VIP2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 1997-12-30 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 53.900, 78.700, 105.900 |
Unit cell angles | 108.65, 89.96, 90.02 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.26900 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.046 | 0.270 |
Total number of observations | 468627 * | |
Number of reflections | 215237 | |
<I/σ(I)> | 13 | |
Completeness [%] | 83.4 | 69.2 |
Redundancy | 2.2 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 20 * | PEG200, BOG, IMIDAZOLE, MALATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | |
3 | 1 | reservoir | PEG200 | 30 (%(w/v)) | |
4 | 1 | reservoir | octyl-beta-D-glucopyranoside | 1 (%(w/v)) | |
5 | 1 | reservoir | imidazole/malate | 200 (mM) |