1QRV

CRYSTAL STRUCTURE OF THE COMPLEX OF HMG-D AND DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1998-08-15
Detector	BRANDEIS - B4
Wavelength(s)	0.9000,0.9211,0.92157,1.05
Spacegroup name	P 21 21 21
Unit cell lengths	43.740, 53.797, 86.843
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.238
Rwork	0.238
R-free	0.28800
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.052	0.198
Total number of observations	94446 *
Number of reflections	10672
<I/σ(I)>	24.2
Completeness [%]	98.0	97.9
Redundancy	8.8	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.85	293 *	Murphy, F.V., (1999) Acta Crystallogr. D55, 1594. *
1	Vapor diffusion *	7.85	293 *	Murphy, F.V., (1999) Acta Crystallogr. D55, 1594. *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 3350,
2	1	1	TRIZMA-CL
3	1	1	NACL
4	1	2	PEG 3350,
5	1	2	TRIZMA-CL
6	1	2	NACL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	5 (mM)
2	1	reservoir	PEG4000	23 (%)
3	1	reservoir	HEPES	10 (mM)