1QRU
GLUTAMINYL-TRNA SYNTHETASE MUTANT I129T COMPLEXED WITH GLUTAMINE TRANSFER RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | AREA DETECTOR |
| Collection date | 1989-12-01 |
| Detector | SDMS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 242.000, 93.700, 115.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 3.000 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.650 |
| Data reduction software | SDMS |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 9.000 * |
| High resolution limit [Å] | 3.000 * |
| Rmerge | 0.097 |
| Number of reflections | 23275 |
| Completeness [%] | 90.7 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 17 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | reservoir | PIPES | 80 (mM) | |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | 0.02 (%) | ||
| 5 | 1 | reservoir | ammonium sulfate | 1.8-2.0 (M) | |
| 6 | 1 | reservoir | ATP | 8 (mM) |
