1QRI
X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE COMPLEXES WITH AN E144D MUTATION AT 2.7 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS-NICOLET X100 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 118.400, 118.400, 49.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.600 |
Rwork | 0.164 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.496 |
Data reduction software | X-GEN |
Data scaling software | X-GEN |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.600 | 2.780 |
High resolution limit [Å] | 2.620 | 2.620 |
Rmerge | 0.072 | 0.133 |
Number of reflections | 7508 | |
Completeness [%] | 61.0 | 10 |
Redundancy | 7 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | PRECIPITANT: 40% PEG 400; 40 MM BTP; PH 8.0; RESERVOIR: 16% PEG 400; 40 MM BTP; PH 6.5; DROP: 5 UL PROTEIN, 2 UL DNA, 1 UL PPT, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 400 | ||
2 | 1 | 1 | BTP | ||
3 | 1 | 1 | PPT | ||
4 | 1 | 2 | PEG 400 | ||
5 | 1 | 2 | BTP |