1QRG
A CLOSER LOOK AND THE ACTIVE SITE OF GAMMA-CARBONIC ANHYDRASES: HIGH RESOLUTION CRYSTALLOGRAPHIC STUDIES OF THE CARBONIC ANHYDRASE FROM METHANOSARCINA THERMOPHILA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-22 |
Detector | MARRESEARCH |
Spacegroup name | P 21 3 |
Unit cell lengths | 82.642, 82.642, 82.642 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.720 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.22700 |
Structure solution method | ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.780 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.097 | 0.240 |
Total number of observations | 128420 * | |
Number of reflections | 19889 | |
<I/σ(I)> | 19 | |
Completeness [%] | 98.0 | 97.6 |
Redundancy | 6.4 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 * | 22 * | PEG 8000, ammonium sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 5 (%) | |
2 | 1 | reservoir | ammonium sulfate | 0.5 (M) |