1QQY
X-RAY CRYSTAL STRUCTURE ANALYSIS OF CANINE MILK LYSOZYME (APO-TYPE)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 277 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-11-25 |
| Detector | WEISSENBERG |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 68.577, 68.577, 107.063 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| Rwork | 0.178 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1EQL |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.414 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.250 |
| Total number of observations | 199512 * | |
| Number of reflections | 13034 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 94.8 | 82.2 |
| Redundancy | 15.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | PEG 4000, tri-sodium citrate, ammonium acetate, EDTA, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium acetate | 0.2 (M) | |
| 3 | 1 | drop | PEG4000 | 30 (%(w/v)) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | trisodium citrate | 0.1 (M) |
