1QQL

THE CRYSTAL STRUCTURE OF FIBROBLAST GROWTH FACTOR 7/1 CHIMERA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	99
Detector technology	CCD
Collection date	1998-12-31
Detector	ADSC QUANTUM 4
Spacegroup name	P 64 2 2
Unit cell lengths	89.800, 89.800, 65.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.237 *
Rwork	0.237
R-free	0.32800
RMSD bond length	0.016
RMSD bond angle	2.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.065	0.299
Total number of observations	64157 *
Number of reflections	7140
<I/σ(I)>	28.1
Completeness [%]	96.8	95
Redundancy	9	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	20 *	SODIUM PHOSPHATE, POTASSIUM PHOSPHATE, SODIUM SULPHATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7	20 *	SODIUM PHOSPHATE, POTASSIUM PHOSPHATE, SODIUM SULPHATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7	20 *	SODIUM PHOSPHATE, POTASSIUM PHOSPHATE, SODIUM SULPHATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)	pH7.0
3	1	drop	ammonium sulfate	20 (mM)
4	1	drop	dithiothreitol	10 (mM)
5	1	drop	EDTA	0.2 (mM)
6	1	reservoir	sodium potassium phosphate	1.75 (M)	pH7.0
7	1	reservoir	dithiothreitol	20 (mM)