1QQH

2.1 A CRYSTAL STRUCTURE OF THE HUMAN PAPILLOMAVIRUS TYPE 18 E2 ACTIVATION DOMAIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	200
Detector technology	CCD
Collection date	1997-12-20
Detector	ADSC QUANTUM 4
Spacegroup name	P 65 2 2
Unit cell lengths	76.240, 76.240, 158.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.256
Rwork	0.256
R-free	0.29700
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.170
High resolution limit [Å]	2.000	2.100
Rmerge	0.052	0.437
Total number of observations	135695 *
Number of reflections	16635
<I/σ(I)>	28.9
Completeness [%]	99.0
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2 *	277	AMMONIUM PHOSPHATE, TRIS, pH 4.0, VAPOR DIFFUSION, temperature 277.0K
1	Vapor diffusion, hanging drop *	8.2 *	277	AMMONIUM PHOSPHATE, TRIS, pH 4.0, VAPOR DIFFUSION, temperature 277.0K
1	Vapor diffusion, hanging drop *	8.2 *	277	AMMONIUM PHOSPHATE, TRIS, pH 4.0, VAPOR DIFFUSION, temperature 277.0K
1	Vapor diffusion, hanging drop *	8.2 *	277	AMMONIUM PHOSPHATE, TRIS, pH 4.0, VAPOR DIFFUSION, temperature 277.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8-12 (mg/ml)
2	1	drop		100 (mM)
3	1	drop	Tris-HCl	25 (mM)
4	1	drop	glycerol	5 (%)
5	1	drop	dithiothreitol	10 (mM)
6	1	reservoir	ammonium phosphate	1.2-1.4 (M)
7	1	reservoir	Tris-HCl	100 (mM)