1QQG
CRYSTAL STRUCTURE OF THE PH-PTB TARGETING REGION OF IRS-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 120.439, 120.439, 79.598 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.24700 |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.380 * |
| Data reduction software | XDS |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.063 | 0.410 |
| Total number of observations | 91581 * | |
| Number of reflections | 29443 * | |
| <I/σ(I)> | 19.94 | |
| Completeness [%] | 97.9 | 98.3 |
| Redundancy | 3.11 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 1.7M SODIUM PHOSPHATE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 21K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 25 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | sodium phosphate | 1.7 (M) | |
| 6 | 1 | reservoir | dithiothreitol | 10 (mM) |
