1QQG

CRYSTAL STRUCTURE OF THE PH-PTB TARGETING REGION OF IRS-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06-01
Detector	MARRESEARCH
Spacegroup name	P 65
Unit cell lengths	120.439, 120.439, 79.598
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.191
Rwork	0.191
R-free	0.24700
RMSD bond length	0.008 *
RMSD bond angle	1.380 *
Data reduction software	XDS
Phasing software	MLPHARE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.063	0.410
Total number of observations	91581 *
Number of reflections	29443 *
<I/σ(I)>	19.94
Completeness [%]	97.9	98.3
Redundancy	3.11	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	1.7M SODIUM PHOSPHATE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 21K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	25 (mM)
3	1	drop		200 (mM)
4	1	drop	dithiothreitol	10 (mM)
5	1	reservoir	sodium phosphate	1.7 (M)
6	1	reservoir	dithiothreitol	10 (mM)