1QQC
CRYSTAL STRUCTURE OF AN ARCHAEBACTERIAL DNA POLYMERASE D.TOK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 1997-12-15 |
| Detector | OTHER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.100, 107.600, 155.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.242 * |
| Rwork | 0.244 |
| R-free | 0.29550 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.616 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.052 | 0.156 |
| Number of reflections | 32909 * | |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 93.9 * | 57.8 |
| Redundancy | 5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 20 * | PEG 400, PEG 4000, AMMONIUM SULFATE, MAGNESIUM SULFATE, TRIS, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 40 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 50 (mM) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 7 | 1 | reservoir | MPD | 20 (%(v/v)) | |
| 8 | 1 | reservoir | PEG4000 | 11 (%(w/v)) | |
| 9 | 1 | reservoir | dithiothreitol | 10 (mM) |
