1QQ9
STREPTOMYCES GRISEUS AMINOPEPTIDASE COMPLEXED WITH METHIONINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.830, 61.830, 146.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.000 - 1.530 |
| R-factor | 0.148 |
| Rwork | 0.148 |
| R-free | 0.17300 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.058 | 0.203 |
| Number of reflections | 42630 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 97.0 | 69.8 |
| Redundancy | 5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 298 | Greenblatt, H.M., (1997) J.Mol.Biol., 265, 620. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 3 | 1 | reservoir | PEG4000 | 16 (%(w/v)) | |
| 4 | 1 | drop | Tris | 10 (mM) | |
| 5 | 1 | drop | 20 (mM) | ||
| 6 | 1 | drop | 1 (mM) |
