1QQ0

COBALT SUBSTITUTED CARBONIC ANHYDRASE FROM METHANOSARCINA THERMOPHILA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-05-05
Detector	MARRESEARCH
Spacegroup name	P 21 3
Unit cell lengths	82.344, 82.344, 82.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.400 - 1.760
R-factor	0.189
Rwork	0.181
R-free	0.21700
RMSD bond length	0.011 *
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.400	1.820
High resolution limit [Å]	1.760	1.760
Rmerge	0.054	0.283
Total number of observations	95188 *
Number of reflections	18449
<I/σ(I)>	21
Completeness [%]	98.7	98.7
Redundancy	5.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2 *	295	PEG 8000, AMMONIUM SULFATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 22K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	phosphate	5 (mM)
3	1	reservoir	PEG8000	5 (%)
4	1	reservoir	ammonium sulfate	0.5 (M)