1QQ0
COBALT SUBSTITUTED CARBONIC ANHYDRASE FROM METHANOSARCINA THERMOPHILA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-05-05 |
Detector | MARRESEARCH |
Spacegroup name | P 21 3 |
Unit cell lengths | 82.344, 82.344, 82.344 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.400 - 1.760 |
R-factor | 0.189 |
Rwork | 0.181 |
R-free | 0.21700 |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.600 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.400 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.054 | 0.283 |
Total number of observations | 95188 * | |
Number of reflections | 18449 | |
<I/σ(I)> | 21 | |
Completeness [%] | 98.7 | 98.7 |
Redundancy | 5.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 * | 295 | PEG 8000, AMMONIUM SULFATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | phosphate | 5 (mM) | |
3 | 1 | reservoir | PEG8000 | 5 (%) | |
4 | 1 | reservoir | ammonium sulfate | 0.5 (M) |