1QPZ
PURINE REPRESSOR-HYPOXANTHINE-PALINDROMIC OPERATOR COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-12 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 176.340, 95.180, 81.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| Rwork | 0.183 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | PROCOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.066 * |
| Total number of observations | 62126 * |
| Number of reflections | 22368 |
| <I/σ(I)> | 9.32 |
| Completeness [%] | 94.0 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.5 (mM) | |
| 10 | 1 | reservoir | PEG4000 | 25 (%) | |
| 11 | 1 | reservoir | ammonium sulphate | 0.4 (M) | |
| 12 | 1 | reservoir | cobalt hexammine | 50 (mM) | |
| 13 | 1 | reservoir | ammonium phosphate | 0.1 (M) | |
| 2 | 1 | drop | dithiothreitol | 1.0 (mM) | |
| 3 | 1 | drop | sodium potassium phosphate | 160 (mM) | |
| 4 | 1 | drop | DNA | 0.6 (mM) | |
| 5 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 6 | 1 | drop | PEG4000 | 25 (%) | |
| 7 | 1 | drop | ammonium sulfate | 0.4 (%) | |
| 8 | 1 | drop | cobalt hexammine | 50 (mM) | |
| 9 | 1 | drop | ammonium phosphate | 0.1 (M) |
