1QPX

CRYSTAL STRUCTURES OF SELF-CAPPING PAPD CHAPERONE HOMODIMERS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-08-25
Detector	RIGAKU RAXIS
Spacegroup name	P 21 21 21
Unit cell lengths	53.065, 75.442, 115.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
Rwork	0.189
R-free	0.26500
RMSD bond length	0.007 *
RMSD bond angle	1.397 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.069	0.270
Total number of observations	107688 *
Number of reflections	18542
<I/σ(I)>	16.7
Completeness [%]	99.3	93.8
Redundancy	5.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	298	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	KMES	20 (mM)
3	1	reservoir	PEG4000	16-22 (%)
4	1	reservoir	isopropyl alcohol	14 (%)
5	1	reservoir	sodium acetate	0.1 (M)