1QPW

CRYSTAL STRUCTURE DETERMINATION OF PORCINE HEMOGLOBIN AT 1.8A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1991-08-10
Detector	RIGAKU RAXIS
Spacegroup name	P 21 21 21
Unit cell lengths	68.100, 72.270, 114.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.211
Rwork	0.207
R-free	0.25100
RMSD bond length	0.017
RMSD bond angle	20.600 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	8.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.058	0.058
Number of reflections	37481 *
<I/σ(I)>	1
Completeness [%]	78.0	78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8	296	2.8M OF PHOSPHATE SOLUTION, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 296K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	80 (mg/ml)
2	1	reservoir	phosphate	2.8 (M)