1QPL

FK506 BINDING PROTEIN (12 KDA, HUMAN) COMPLEX WITH L-707,587

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1991-06-30
Detector	SIEMENS
Spacegroup name	I 4
Unit cell lengths	119.740, 119.740, 57.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.223
Rwork	0.223
R-free	0.34200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PROTEIN PORTION OF PDB ENTRY 1FKD
RMSD bond length	0.007
RMSD bond angle	26.200 *
Data scaling software	X-GEN
Phasing software	MERLOT
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.330	2.610
High resolution limit [Å]	2.500	2.500
Rmerge	0.059
Number of reflections	12979
<I/σ(I)>	29.28
Completeness [%]	85.1	57.2
Redundancy	6.41

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.1		AMMONIUM SULFATE, POTASSIUM PHOSPHATE, pH 6.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	39 (%sat)
2	1	reservoir	potassium phosphate	0.10 (M)