1QPK

MUTANT (D193G) MALTOTETRAOSE-FORMING EXO-AMYLASE IN COMPLEX WITH MALTOTETRAOSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	63.210, 167.500, 46.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.178
RMSD bond length	0.014
RMSD bond angle	0.034
Phasing software	PHASER
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	2.000
Rmerge	0.082
Total number of observations	99872 *
Number of reflections	31354
Completeness [%]	90.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	20 *	AMMONIUM SULFATE, TRIS-HCL, pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop	ammonium sulfate	0.7-0.9 (M)
4	1	reservoir	maltopentaose	10 (mg/ml)