Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-02 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.049, 119.700, 81.283 |
| Unit cell angles | 90.00, 120.21, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pvd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.108 | 0.350 |
| Number of reflections | 42140 | |
| <I/σ(I)> | 12 | 5 |
| Completeness [%] | 88.9 | 56 * |
| Redundancy | 4.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 8-14% PEG6000 20MM SODIUM CITRATE AT PH 5.7 1MM DTE, 5MM THDP, 5MM MGSO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | 5 (mM) | ||
| 11 | 1 | reservoir | PEG6000 | 13-14 (%) | |
| 2 | 1 | drop | sodium citrate | 20 (mM) | |
| 3 | 1 | drop | DTE | 1 (mM) | |
| 4 | 1 | drop | ThDP | 5 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | pyruvamide | 320 (mM) | |
| 7 | 1 | reservoir | sodium citrate | 20 (mM) | |
| 8 | 1 | reservoir | DTE | 1 (mM) | |
| 9 | 1 | reservoir | ThDP | 5 (mM) |
