1QOV
PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ALA M260 REPLACED WITH TRP (CHAIN M, A260W)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 1998-03-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 142.050, 142.050, 186.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| Rwork | 0.169 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD-TYPE RHODOBACTER SPHAEROIDES COORDINATES (UNPUBLISHED DATA) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.057 | 0.266 |
| Number of reflections | 124853 | |
| Completeness [%] | 97.6 | 78.6 |
| Redundancy | 4.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 | pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | reaction center | 10 (mg/ml) | |
| 2 | 1 | drop | LDAO | 0.1 (%(v/v)) | |
| 3 | 1 | drop | 1,2,3-heptanetriol | 3.5 (%(w/v)) | |
| 4 | 1 | drop | trisodium citrate | 0.5 (M) | |
| 5 | 1 | drop | Tris-HCl | 10 (mM) | |
| 6 | 1 | reservoir | trisodium citrate | 1.1 (M) |
