1QOV
PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ALA M260 REPLACED WITH TRP (CHAIN M, A260W)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 1998-03-15 |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 142.050, 142.050, 186.810 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
Rwork | 0.169 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | WILD-TYPE RHODOBACTER SPHAEROIDES COORDINATES (UNPUBLISHED DATA) |
RMSD bond length | 0.011 |
RMSD bond angle | 0.026 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.057 | 0.266 |
Number of reflections | 124853 | |
Completeness [%] | 97.6 | 78.6 |
Redundancy | 4.2 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8 | pH 8.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | reaction center | 10 (mg/ml) | |
2 | 1 | drop | LDAO | 0.1 (%(v/v)) | |
3 | 1 | drop | 1,2,3-heptanetriol | 3.5 (%(w/v)) | |
4 | 1 | drop | trisodium citrate | 0.5 (M) | |
5 | 1 | drop | Tris-HCl | 10 (mM) | |
6 | 1 | reservoir | trisodium citrate | 1.1 (M) |