1QOS
lectin UEA-II complexed with chitobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 106.030, 106.030, 87.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.950 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UEA-II COMPLEXED WITH NAG (1QOO) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 28.896 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.080 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.135 | 0.550 |
| Total number of observations | 47378 * | |
| Number of reflections | 12117 | 1200 * |
| <I/σ(I)> | 10.18 | 3.24 |
| Completeness [%] | 98.2 | 99.1 |
| Redundancy | 3.91 | 4.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | Dao-Thi M.H., (1998) Acta Crystallog. sect., D54, 844. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.0-5.0 (mg/ml) | |
| 2 | 1 | reservoir | cacodylate | 100 (mM) | or MES |
| 3 | 1 | reservoir | PEG6000 | 8-16 (%(w/v)) |
