1QOR

CRYSTAL STRUCTURE OF ESCHERICHIA COLI QUINONE OXIDOREDUCTASE COMPLEXED WITH NADPH

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	107.440, 104.060, 77.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 * - 2.200
R-factor	0.14
Rwork	0.140
RMSD bond length	0.015
RMSD bond angle	25.187 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.073 *
Number of reflections	40569 *
Completeness [%]	90.6 *	75.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	23 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	EDTA	0.1 (mM)
4	1	drop	beta-mercaptoethanol	1 (mM)
5	1	drop	NADPH	14 (mM)
6	1	reservoir	ammonium sulfate	1.75-2.25 (M)