1QOL
STRUCTURE OF THE FMDV LEADER PROTEASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.430, 101.560, 276.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 3.000 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.103 * | 0.263 * |
| Number of reflections | 35080 | |
| <I/σ(I)> | 9.5 | 2.9 |
| Completeness [%] | 92.5 | 75 * |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | 10% PEG 6000, 0.8 M MG CHLORIDE, 0.1 M TRIS/HCL PH=8.5, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 10 (%) | |
| 2 | 1 | reservoir | 0.8 (M) | ||
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) |
