1QOI
U4/U6 snRNP-specific cyclophilin SnuCyp-20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.300, 59.900, 60.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cpl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 * | 0.115 * |
| Total number of observations | 44280 * | |
| Number of reflections | 12102 | |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 97.8 | 98.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 * | 21 * | 25% PEG6000, 200 MM MGCL2, 100 MM TRISHCL, PH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 120 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | HEPES | 20 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 25 (%(w/v)) | |
| 6 | 1 | reservoir | 200 (mM) | ||
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) |
