1QO4
ARABIDOPSIS THALIANA PEROXIDASE A2 AT ROOM TEMPERATURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.290, 74.848, 80.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pa2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.165 * | 0.295 * |
| Total number of observations | 27478 * | |
| Number of reflections | 5887 | |
| <I/σ(I)> | 8 | 5 |
| Completeness [%] | 97.8 * | 97.3 |
| Redundancy | 4.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | Ostergaard, L., (2000) Plant Mol. Biol., 44, 231. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.98 (mg/ml) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | drop | cacodylate | 0.5 (M) | |
| 4 | 1 | drop | PEG8000 | 10 (%(w/v)) | |
| 5 | 1 | reservoir | 0.2 (M) | ||
| 6 | 1 | reservoir | cacodylate | 0.1 (M) | |
| 7 | 1 | reservoir | PEG8000 | 20 (%(w/v)) |
