1QO2
Crystal structure of N-((5'-phosphoribosyl)-formimino)-5-aminoimidazol-4-carboxamid ribonucleotid isomerase (EC 3.1.3.15, HisA)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97893, 0.97929, 0.98089 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.780, 73.160, 62.700 |
| Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.24600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.310 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.9) |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.841 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.048 * | 0.304 * |
| Number of reflections | 34932 | |
| <I/σ(I)> | 17 | 2.53 |
| Completeness [%] | 97.4 | 91.9 |
| Redundancy | 5.8 * | 1.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 20 * | Thoma, R., (1999) FEBS Lett., 454, 1. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | Bis-Tris | 10 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 29-35 (%(w/v)) | |
| 6 | 1 | reservoir | citrate | 0.1 (M) | |
| 7 | 1 | reservoir | 200 (mM) | ||
| 8 | 1 | reservoir | MPD | 3 (%(v/v)) |
