1QO0
Amide receptor of the amidase operon of Pseudomonas aeruginosa (AmiC) complexed with the negative regulator AmiR.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-07-15 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 308.440, 67.150, 76.410 |
Unit cell angles | 90.00, 103.33, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.250 |
Rwork | 0.186 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pea |
RMSD bond length | 0.010 |
RMSD bond angle | 0.034 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.600 | 2.360 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 67099 | |
<I/σ(I)> | 6.8 | 3 |
Completeness [%] | 83.8 | 69.3 |
Redundancy | 2.7 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.6 | CRYSTALLISATION WAS BY MICRO-BATCH AND STREAK SEEDING. WELLS CONTAINED 8-8.5% PEG4000, 20%(V/V) 2-PROPANOL, 50MM SODIUM CITRATE BUFFERED AT PH 5.6 AND AMIC-AMIR COMPLEX AT 5MG/ML (FINAL CONCENTRATION). |