1QNX
Ves v 5, an allergen from Vespula vulgaris venom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.359, 62.179, 82.777 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.23600 |
Structure solution method | MIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 23.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (0.5) |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.067 * | 0.178 * |
Number of reflections | 17809 | |
Completeness [%] | 93.2 | 98.9 |
Redundancy | 8.1 | 3.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5.6 | 18% PEG6000, 0.1M CITRTE BUFFER PH 5.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 16-20 (%(w/v)) | |
2 | 1 | reservoir | citrate | 100 (mM) | |
3 | 1 | drop | protein | 5 (mg/ml) |