1QNP

The 3-D structure of a Trichoderma reesei b-mannanase from glycoside hydrolase family 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	110
Detector technology	CCD
Detector	ADSC QUANTUM
Spacegroup name	P 1 21 1
Unit cell lengths	50.180, 54.600, 60.760
Unit cell angles	90.00, 111.23, 90.00

Refinement procedure

Resolution	20.000 - 1.500
Rwork	0.127
R-free	0.17400
Structure solution method	OTHER
RMSD bond length	0.016
RMSD bond angle	0.031
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.063 *	0.063
Number of reflections	48906
<I/σ(I)>	20.9	20.9
Completeness [%]	99.8	95.4
Redundancy	3.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	293 *	2M AMMONIUM SULPHATE, 0.1M GLYCINE PH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	22 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	ammonium sulfate	2 (M)
4	1	reservoir	Tris-HCl	0.1 (M)