1QNO

The 3-D structure of a Trichoderma reesei b-mannanase from glycoside hydrolase family 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	290
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	50.010, 54.270, 60.240
Unit cell angles	90.00, 111.31, 90.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.126
R-free	0.20000
Structure solution method	OTHER
RMSD bond length	0.015
RMSD bond angle	0.034
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.030 *	0.030
Number of reflections	19562
<I/σ(I)>	42.8	20.8
Completeness [%]	97.8	74.6
Redundancy	5.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	293 *	2M AMMONIUM SULPHATE 0.1M TRIS-HCL PH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	22 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	ammonium sulfate	2 (M)
4	1	reservoir	Tris-HCl	0.1 (M)