1QNO
The 3-D structure of a Trichoderma reesei b-mannanase from glycoside hydrolase family 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.010, 54.270, 60.240 |
| Unit cell angles | 90.00, 111.31, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.126 |
| R-free | 0.20000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.030 * | 0.030 |
| Number of reflections | 19562 | |
| <I/σ(I)> | 42.8 | 20.8 |
| Completeness [%] | 97.8 | 74.6 |
| Redundancy | 5.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 293 * | 2M AMMONIUM SULPHATE 0.1M TRIS-HCL PH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 22 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) |
