1QNM
HUMAN MANGANESE SUPEROXIDE DISMUTASE MUTANT Q143N
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 90 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-05 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.500, 78.600, 67.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.300 |
| R-factor | 0.198 * |
| Rwork | 0.199 |
| R-free | 0.29400 * |
| Structure solution method | ISOMORPHOUS REFINEMENT |
| Starting model (for MR) | 1ABM |
| RMSD bond length | 0.011 |
| RMSD bond angle | 23.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 * | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.092 * | |
| Total number of observations | 55987 * | |
| Number of reflections | 18015 | |
| <I/σ(I)> | 8.4 | 3 |
| Completeness [%] | 96.4 | 80 * |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | or sitting drop method * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 40 (mM) | |
| 3 | 1 | reservoir | mPEG2000 | 20 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 5 | 1 | reservoir | 1,2,3-heptanetriol | 0.8 (%) |
