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1QNE

Crystal structure of the Adenovirus major late promoter TATA box bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2).

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]100
Detector technologyAREA DETECTOR
Spacegroup nameP 1 21 1
Unit cell lengths42.000, 147.000, 57.000
Unit cell angles90.00, 90.50, 90.00
Refinement procedure
Resolution6.000 - 1.900
R-factor0.197
Rwork0.197
R-free0.26500
Structure solution methodOTHER
RMSD bond length0.013
RMSD bond angle1.700
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareX-PLOR
Refinement softwareX-PLOR
Data quality characteristics
 Overall
Low resolution limit [Å]20.000
High resolution limit [Å]1.880
Rmerge0.052

*

Number of reflections51793
Completeness [%]93.2
Redundancy3.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

6.2

*

4

*

pH 6.00
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirglycerol14 (%(v/v))
21reservoirdithiothreitol15 (mM)
31reservoirMES25 (mM)

227344

PDB entries from 2024-11-13

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