1QNE
Crystal structure of the Adenovirus major late promoter TATA box bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.000, 147.000, 57.000 |
Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.900 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.26500 |
Structure solution method | OTHER |
RMSD bond length | 0.013 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.880 |
Rmerge | 0.052 * |
Number of reflections | 51793 |
Completeness [%] | 93.2 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.2 * | 4 * | pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | glycerol | 14 (%(v/v)) | |
2 | 1 | reservoir | dithiothreitol | 15 (mM) | |
3 | 1 | reservoir | MES | 25 (mM) |