1QN4
Crystal structure of the T(-24) Adenovirus major late promoter TATA box variant bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2). TATA element recognition by the TATA box-binding protein has been conserved throughout evolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.000, 57.000, 147.000 |
Unit cell angles | 90.00, 96.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.860 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PLANT TBP-ADMLP TATA DNA |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 1.860 |
Rmerge | 0.042 * |
Number of reflections | 57768 |
Completeness [%] | 96.5 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5.9 * | 4 * | pH 6.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | complex | 0.5 (mM) | |
2 | 1 | drop | MES | 40 (mM) | |
3 | 1 | drop | 60 (mM) | or 100 mM | |
4 | 1 | drop | 4 (mM) | ||
5 | 1 | drop | glycerol | 14 (%(v/v)) | |
6 | 1 | drop | ammonium acetate | 300 (mM) | |
7 | 1 | drop | dithiothreitol | 10 (mM) | |
8 | 1 | reservoir | MES | 12 (%(v/v)) | |
9 | 1 | reservoir | dithiothreitol | 10 (mM) |